cpt2 (Proteintech)
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Proteintech
cpt2
Cpt2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cpt2/CPT1B-specific+Antibody/pmc12989855-523-19-17
Average 96 stars, based on 136 article reviews
Cpt2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cpt2/CPT1B-specific+Antibody/pmc12989855-523-19-17
Average 96 stars, based on 136 article reviews
cpt2 - by Bioz Stars,
2026-11
96/100 stars
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Blocking Assay:Article Title: Cardiac PTN-SIRT1 axis alleviates oxidative stress and promotes mitochondrial energy reprogramming to mitigate doxorubicin-induced cardiotoxicity through AMPK/PGC1α signaling. Article Snippet: Following measurement of levels with BCA assays (Beyotime), proteins were electrophoresed on SDS-PAGE followed by PVDF membrane transfer. .. After blocking (5% non-fat milk or BSA in TBST), membranes were treated overnight at 4°C with the following primary antibodies: anti-PTN (27117-1-AP; Proteintech, Wuhan, China), anti-GAPDH (60004-1-Ig; Proteintech), anti-Bax (50599-2- Ig; Proteintech), anti-Bcl-2 (12789-1-AP; Proteintech), anti-Flag (PM020; anti-MBL, Beijing, China), anti-SIRT1 (13161-1-AP; Proteintech), anti-SIRT2 (19655-1-AP; Proteintech), anti-AMPK (10929-2-AP; Proteintech), anti-p-AMPK (Thr172) (2535S; CST, Boston, USA), anti-PGC1α (66369-1-Ig; Proteintech), anti-CPT1 (15184-1-AP; Proteintech), and Article Title: Cardiac PTN-SIRT1 axis alleviates oxidative stress and promotes mitochondrial energy reprogramming to mitigate doxorubicin-induced cardiotoxicity through AMPK/PGC1α signaling Article Snippet: Following measurement of levels with BCA assays (Beyotime), proteins were electrophoresed on SDS-PAGE followed by PVDF membrane transfer. .. After blocking (5% non-fat milk or BSA in TBST), membranes were treated overnight at 4°C with the following primary antibodies: anti-PTN (27117-1-AP; Proteintech, Wuhan, China), anti-GAPDH (60004-1-Ig; Proteintech), anti-Bax (50599-2-Ig; Proteintech), anti-Bcl-2 (12789-1-AP; Proteintech), anti-Flag (PM020; anti-MBL, Beijing, China), anti-SIRT1 (13161-1-AP; Proteintech), anti-SIRT2 (19655-1-AP; Proteintech), anti-AMPK (10929-2-AP; Proteintech), anti-p-AMPK (Thr172) (2535S; CST, Boston, USA), anti-PGC1α (66369-1-Ig; Proteintech), anti-CPT1 (15184-1-AP; Proteintech), and other:Article Title: Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation. Article Snippet: In brief Guo et al. demonstrate that CCN1’s mitochondrial translocation is critical for ferroptosis induction through the formation of the CCN1-ETFA complex, which facilitates abnormal fatty acid oxidation.. Additionally, they highlight the potential of a high-fat diet or hypertriglyceridemia to enhance the efficacy of ferroptosis therapy in lung carcinoma.. CPT2 ETFA CPT2 ETFA CCN1 Normal condition Ferroptosis induction Immunoprecipitation:Article Title: SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction. Article Snippet: The supernatants were collected and protein concentrations were assessed using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. For immunoprecipitation, 1 mg of protein sample was incubated with 5 μl of Article Title: SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction Article Snippet: The supernatants were collected and protein concentrations were assessed using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. For immunoprecipitation, 1 mg of protein sample was incubated with 5 μl of Incubation:Article Title: SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction. Article Snippet: The supernatants were collected and protein concentrations were assessed using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. For immunoprecipitation, 1 mg of protein sample was incubated with 5 μl of Article Title: Immunoregulatory electrospinning fiber mediates Macrophage energy metabolism reprogramming to promote burn wound healing Article Snippet: Skin tissues and BMDM cells were collected and homogenised with RIPA lysis buffer (BL504A, Biosharp, China) and then sonicated with an ultrasonograph (Scientz-48L, Xinzhi, China). .. The protein-containing PVDF membranes were blocked with 5 % skimmed milk and then the PVDF membranes were blocked with various primary antibodies including anti-CD206 (ab300621, Abcam, UK), anti-CD86 (CY5238, Abways, China), anti-PPARγ (41360, SAB, USA), anti-iNOS (ab178945, Abcam, UK), anti-Arg1 (A25808, Abclonal, China), Article Title: SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction Article Snippet: The supernatants were collected and protein concentrations were assessed using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. For immunoprecipitation, 1 mg of protein sample was incubated with 5 μl of Gentle:Article Title: SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction. Article Snippet: The supernatants were collected and protein concentrations were assessed using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. For immunoprecipitation, 1 mg of protein sample was incubated with 5 μl of Article Title: SLC44A2 negatively regulates mitochondrial fatty acid oxidation to suppress colorectal progression by blocking the MUL1-CPT2 interaction Article Snippet: The supernatants were collected and protein concentrations were assessed using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. For immunoprecipitation, 1 mg of protein sample was incubated with 5 μl of Western Blot:Article Title: Qifu Decoction Alleviates Lipopolysaccharide-Induced Myocardial Dysfunction by Inhibiting TLR4/NF-κB/NLRP3 Inflammatory Pathway and Activating PPARα/CPT Pathway Article Snippet: An LDH assay kit was purchased from Nanjing Jiancheng Bioengineering institute Co., Ltd. (Nanjing, China). .. The antibodies used for Western blot in this study were as follows: anti-NLRP3 (1:1000, ab263899, Abcam, Waltham, MA, USA), anti-TLR4 (1:1000, 14358, Cell Signaling Technology, Danvers, MA, USA), anti-COX2 (1:1000, ab62331, Abcam, Waltham, MA, USA), anti-TNF-α (1:1000, 17590-1-AP, Proteintech, Rosemont, IL, USA), anti-sPLA2 (1:1000, M02259-1, BOSTER, Hangzhou, China), anti-IL-6 (1:1000, DF6087, Affinity Biosciences, Cincinnati, OH, USA), anti-NF-κB p65 (1:1000, 8242, Cell Signaling Technology, Danvers, MA, USA), anti-p-NF-κB p65 (1:1000, 3033, Cell Signaling Technology, Danvers, MA, USA), anti-β-actin (1:500, AP0060, Bioworld, Visalia, CA, USA), |